LUYOR-3415用于觀察小鼠黑色素瘤轉(zhuǎn)移

2022年10月重慶醫(yī)科大學(xué)在《Biological Research》發(fā)表《Melanoma stem cells promote metastasis via exosomal miR-1268a inactivation of autophagy》文獻，文獻中實驗采用了LUYOR-3415RG雙波長熒光蛋白激發(fā)光源和LUV-50A熒光觀察眼鏡觀察紅色熒光蛋白RFP在小鼠肺部腫瘤細胞的上的表達。

To study the effect of miR-1268a overexpression on OL cells metastasis in vivo, we generated OL cells stably overexpressing miR-1268a via lentiviral infection (Fig. 4J). 5 次 10^{5} OE-NC-OL and OE-miR-1268a-OL cells were injected into NOD/SCID mice via the tail vein, respectively (Sect. “Materials and methods”). All mice were sacrificed at day 35 after tumor cell injection. Since OE-NC-OL and OE-miR-1268a-OL cells were RFP-labeled, macroscopic metastatic foci formed at the lung could be visualize under external fluorescence imaging using LUYOR-3415 Dual Fluorescent Protein Excitation Light Source and LUV-50A Glasses. As shown in Fig. 4K, injected OE-miR-1268a-OL cells produced significantly more macroscopic lung foci than the OE-NC-OL cells (n?=?6). Subsequently, H&E analysis was performed on the largest cross-sectional lung sections of the two groups of mice. Statistical analysis showed that there were significantly more microscopic metastatic foci in the lungs of mice treated with OE-miR-1268a-OL cells (The black arrows show the formed metastases) (Fig. 4L). These observations show that miR-1268a can enhance metastatic colonization efficiency of OL cells that are oligometastatic. So far, we have demonstrated that miR-1268a, transferred from OL-SCs-derived exosomes to OL cells, can effectively augment the clonogenic colonization capability of OL cells to give arise to more extensive macroscopic metastases.

文獻地址：https://biolres.biomedcentral.com/articles/10.1186/s40659-022-00397-z 

上海峰志儀器有限公司現(xiàn)貨供應(yīng)美國路陽生產(chǎn)的LUYOR-3415RG手持式雙波長熒光蛋白激發(fā)光源，能夠觀察綠色熒光蛋白和1紅色熒光蛋白在小鼠、斑馬魚、線蟲等模式動物上的表達。

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重慶醫(yī)科大學(xué)介紹：

重慶醫(yī)科大學(xué)創(chuàng)建于1956年，由上海醫(yī)學(xué)院（現(xiàn)復(fù)旦大學(xué)上海醫(yī)學(xué)院）分遷來渝組建，原名重慶醫(yī)學(xué)院，1985年更名為重慶醫(yī)科大學(xué)。學(xué)校是國務(wù)院學(xué)位委員會批準(zhǔn)的首批具有博士和碩士學(xué)位授予權(quán)的單位，是教育部批準(zhǔn)的首批來華留學(xué)示范基地。學(xué)校2015年成為重慶市人民政府、衛(wèi)生健康委員會（原衛(wèi)生計生委）和教育部共建高校，2016年成為“高等學(xué)校學(xué)科創(chuàng)新引智計劃”（“111計劃”）首批立項的地方高校之一。學(xué)校由位于渝中區(qū)的袁家崗校區(qū)和位于重慶大學(xué)城的縉云校區(qū)組成，校園總面積2650畝，其中縉云校區(qū)占地2100畝。校園環(huán)境優(yōu)美，風(fēng)景如畫。

重慶醫(yī)科大學(xué)現(xiàn)有全日制在校學(xué)生26810人，其中博士、碩士研究生7124人，本科生18824人，?？粕?58人（對口支援少數(shù)民族項目），留學(xué)生604人。學(xué)校具有招收博士后研究人員，港澳臺學(xué)生和外國留學(xué)生資格。學(xué)?，F(xiàn)有8個一級學(xué)科博士學(xué)位授權(quán)點、13個一級學(xué)科碩士學(xué)位授權(quán)點，3個博士專業(yè)學(xué)位授權(quán)點、11個碩士專業(yè)學(xué)位授權(quán)點；4個重點學(xué)科，8個學(xué)科進入ESI全球前1%（2022年1月科睿唯安數(shù)據(jù)），4個重慶市學(xué)科，16個重慶市“十四五”市級重點學(xué)科。

文章轉(zhuǎn)自：https://www.luyoruv.com/news/news1/673.html