熒光適配器用于樣品的預篩選

熒光適配器用于樣品的預篩選

美國NIGHTSEA SFA-RB體視顯微鏡熒光適配器可以將您的常規(guī)實驗室體視顯微鏡變成一種有價值的工具，用于在進入更高分辨率的熒光顯微鏡或共聚焦顯微鏡之前預先篩選樣品制備的熒光。上海峰志儀器有限公司代理銷售美國nightsea體視顯微鏡熒光適配器和熒光手電筒。有關(guān)體視顯微鏡熒光適配器產(chǎn)品介紹請瀏覽《美國nightsea熒光適配器 》，上海峰志儀器有限公司銷售的熒光觀察設(shè)備能夠觀察植物愈傷、葉片、種子、根系等上面的轉(zhuǎn)基因的表達，還能觀察轉(zhuǎn)基因在動物如老鼠、斑馬魚、果蠅等實驗動物上的表達。有便攜式單波長熒光手電筒，也有手持式大面積照射高強度的雙波長激發(fā)光源，上海峰志提供免費試機，有興趣的老師和同學可按照網(wǎng)頁底部聯(lián)系聯(lián)系。

挑戰(zhàn)

生物樣品的高分辨率成像主要基于熒光技術(shù)。共聚焦、雙光子和高分辨率復合熒光顯微鏡幾乎總是有限的資源。它們通常僅位于成像核心設(shè)施中，并且可按計劃按使用付費。將熒光團引入樣品的過程并不總是成功的。染色，將含GFP的質(zhì)粒引入細胞，免疫組織化學 - 這些都是錯誤的。花時間在高端系統(tǒng)上尋找熒光并不罕見，而那里甚至沒有發(fā)現(xiàn)任何熒光。

實用的解決方案

美國NIGHTSEA SFA-RB可以在標準體視顯微鏡上對標本進行熒光預篩選。您看到的細節(jié)并不重要 - 熒光的簡單存在或不存在以及一般位置可以讓您知道是否值得將標本帶到成像核心。在使用費的直接費用和浪費在查看非熒光樣品的時間之間，NIGHTSEA系統(tǒng)不需要很多節(jié)省的旅行來支付自己。

一位研究人員的工作要求用Alexa Fluor 488 Phalloidin染色兔子腰大肌纖維。對于沒有沾染污漬的樣品，有些令人沮喪。在獲得SFA-RB熒光適配器后，她寫道：

"利用NIGHTSEA SFA-RB體視顯微鏡熒光適配器預篩選是一種很好的方法，可以在我們嘗試在共聚焦上以更高的放大倍率對肌肉纖維進行成像之前快速檢查染色是否成功。

兔腰大肌纖維在白光和熒光下用Alexa Fluor 488 Phalloidin染色。使用NIGHTSEA熒光適配器的白光LED（左）和皇家藍激發(fā)（SFA-RB）/發(fā)射光+濾光片組制作的圖像。樣本由布朗大學的Beth Brainerd博士和Natividad Chen博士提供。

另一位研究人員使用斑馬魚作為一個系統(tǒng)來研究不同的有毒物質(zhì)（藥物，農(nóng)藥，食品添加劑等）如何改變大腦發(fā)育。他寫道：

"在使用NIGHTSEA SFA-RB熒光適配器篩選樣品之前，我必須選擇要安裝的樣品，去共聚焦，然后希望我的一些樣品實際上是熒光的?，F(xiàn)在我使用NIGHTSEA體視顯微鏡熒光適配器來預篩選我的樣品，通過確保我成像的樣品是熒光的，我節(jié)省了時間和金錢。

轉(zhuǎn)基因斑馬魚大腦共聚焦圖像

轉(zhuǎn)基因斑馬魚（Dania rerio）大腦的共聚焦圖像。Kaede蛋白 – 綠色是未轉(zhuǎn)換的，紅色是光轉(zhuǎn)換的。圖片由布朗大學Creton Lab的Robert Thorn提供。

英文原文：

Pre-Screening Samples for Fluorescence

The NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter can turn your routine laboratory stereo microscope into a valuable tool for pre-screening your sample preparations for fluorescence before moving on to higher resolution systems.

The Challenge

High resolution imaging of biological samples is heavily based on fluorescence techniques. Confocal, 2-photon, and high resolution compound fluorescence microscopes are almost always a limited resource. They are often located only in imaging core facilities and accessible on a scheduled, pay-per-use basis.

The processes for introducing fluorophores to specimens are not always successful. Staining, introduction of GFP-bearing plasmids to cells, immunohistochemistry – all are fallible. It is not unusual to spend time searching for fluorescence on a high end system when there is not even any there to be found.

The Practical Solution

The NIGHTSEA SFA enables fluorescence pre-screening of specimens on a standard stereo microscope. The detail that you see is not important – the simple presence or absence and general location of fluorescence lets you know whether it is worth taking your specimen to the imaging core. Between the direct expense of the use fee and the time wasted to look at a non-fluorescent specimen it will not take many saved trips for the NIGHTSEA system to more than pay for itself.

One researcher’s work requires staining rabbit psoas muscle fibers with Alexa Fluor 488 Phalloidin. There was some frustration with samples that did not take up the stain. After acquiring the SFA she wrote:

“The NIGHTSEA fluorescence setup is a great way to quickly check whether the stain was successful before we try to image the muscle fiber at a higher magnification on the confocal.”

Rabbit psoas muscle fibers stained with Alexa Fluor 488 Phalloidin

Rabbit psoas muscle fibers stained with Alexa Fluor 488 Phalloidin, in white light and fluorescence. Images made using NIGHTSEA’s white LED (left) and the Royal Blue excitation/emission light+filter set. Samples courtesy of Dr. Beth Brainerd and Natividad Chen, Brown University.

Another researcher uses zebrafish as a system to look at the way different toxicants (pharmaceuticals, pesticides, food additives, etc.) alter brain development. He writes:

“Before using NIGHTSEA to screen my samples, I would have to select samples to mount, go to the confocal and then hope that some of my samples were actually fluorescent. Now that I use NIGHTSEA to prescreen my samples I save both time and money by making sure the only samples I image are fluorescent.”

Confocal image of brain of transgenic zebrafish

Confocal image of brain of transgenic zebrafish (Dania rerio). Kaede protein – green is unconverted, red is photoconverted. Image courtesy of Robert Thorn, Creton Lab, Brown University.